human muscle growth medium  (PromoCell)

Journal: bioRxiv

Article Title: Development of high-affinity, single-domain protein binders for neutralizing household allergens

doi: 10.1101/2025.08.03.668213

Figure Lengend Snippet: (a) Thermostability profile of AVA-1-1-C3 as a representative protein, as determined by differential scanning fluorimetry. (b-g) Cytotoxicity of primary human cells following 48-hour exposure to AVA-1-1-C3 or an equivalent concentration of bovine serum albumin (BSA), evaluated using the CellTiter-Glo® (CTG) luminescent cell viability assay. Cell types included human epithelial keratinocytes (HEK, b), human dermal fibroblasts (HDF, c), human follicle dermal papilla cells (HFDPC, d), human renal cortical epithelial cells (HRCEPC, e), human skeletal muscle cells (HSKMC, f), and human dermal microvascular endothelial cells (HDMEC, g). No significant differences in cell viability were observed. Data in (b-g) represents mean ± s.d. and are representative of two experimental replicates.

Article Snippet: Cells were cultured in their corresponding complete media including HDF growth medium (Cell Applications, Cat# 116-500), human EpiVita serum-free growth medium (Cell Applications, Cat# 141-500a), microvascular endothelial cell growth medium (PromoCells, Cat# C-22120), HSkMC growth medium (Cell Applications, Cat# 151-500), and renal epithelial cell growth media (PromoCell, Cat# C-26130).

Techniques: Concentration Assay, Cell Viability Assay

Journal: bioRxiv

Article Title: CRISPR-Cas9 mediated endogenous utrophin upregulation improves Duchenne Muscular Dystrophy

doi: 10.1101/2023.04.18.536394

Figure Lengend Snippet: (A) Schematic representation of the timeline for generating 3D mini muscle starting from treated or untreated myoblasts. (B) TIDE quantification of the indels generated by the gRNA Let-7c-1 in 3D hDMD mini-muscle. Bars represent mean ± SEM of n = 3 per condition. Dots represent each experiment. (C) Indel profiles generated by the gRNA-hLet-7c-1 in 3D hDMD mini-muscle. In the inset are indicated the % at which each nucleotide is inserted in the +1 indels. Bars are mean ± SEM of n = 3 per condition. (D) Quantitative PCR analysis of utrophin A mRNA expression level, normalized with gapdh, after editing with the indicated gRNAs in 3D hDMD mini-muscle. Bars are mean ± SEM of n = 3 per condition. Dots represent each experiment. *p < 0.05. (E) Relative utrophin protein expression in 3D DMD muscles after editing with the indicated gRNA was determined by western blot and standardized for GAPDH loading. Bar graph: WB quantification. Bars represent mean ± SEM of n = 2 per condition. Dots represent each experiment. (F) Immunofluorescence staining for utrophin and myosin heavy chain (MyHC) on transverse sections of 3D DMD muscles show a higher utrophin signal after treatment with gRNA-hLet-7c compared to control. n = 4 per group. Scale bar: 100 µm. (G) Quantification of the immunostaining shown in (F) . Bars represent mean ± SEM of n = 3 per condition. Dots represent each experiment.

Article Snippet: Human DMD myoblasts were maintained in Smooth Muscle Cell Growth Medium (C-23060, PromoCell) supplemented with 20% fetal bovine serum gold (PAA) and 1% penicillin– streptomycin (Invitrogen).

Techniques: Generated, Real-time Polymerase Chain Reaction, Expressing, Muscles, Western Blot, Immunofluorescence, Staining, Control, Immunostaining